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ATCC mediterranei atcc 33500 reference genome jo
Mediterranei Atcc 33500 Reference Genome Jo, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mediterranei+genome/Haloferax+mediterranei/pm40250665-79-8-9
Average 96 stars, based on 52 article reviews

mediterranei atcc 33500 reference genome jo - by Bioz Stars, 2026-09

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Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation.
Article Snippet: The aim of this work is to develop bioinformatics research as the first step for further omics-based studies to shed light on copper metabolism in haloarchaea by analyzing H. mediterranei genome (strain ATCC 33500).

Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation
Article Snippet: The aim of this work is to develop bioinformatics research as the first step for further omics-based studies to shed light on copper metabolism in haloarchaea by analyzing H. mediterranei genome (strain ATCC 33500).

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Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation
Article Snippet: .. Regarding the bioinformatic analysis of H. mediterranei genome (strain ATCC 33500), the list of functional genes available in HaloWeb was reviewed. ..

Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation.
Article Snippet: .. Regarding the bioinformatic analysis of H. mediterranei genome (strain ATCC 33500), the list of functional genes available in HaloWeb2 was reviewed. ..

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Strains of H. mediterranei used in reporter gene assays.

Journal: Frontiers in Microbiology

Article Title: Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei

doi: 10.3389/fmicb.2023.1109550

Figure Lengend Snippet: Strains of H. mediterranei used in reporter gene assays.

Article Snippet: Promoter regions of the studied genes ( ; , ) were amplified by PCR from H. mediterranei R4 ATCC 33500 genomic DNA (genome consists of one chromosome of 2,948,887 bp and three megaplasmids, pHME132 of 131.975 bp, pHME322 of 321.907 bp, and pHME505 of 504,704 bp) ( ; ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei

doi: 10.3389/fmicb.2023.1109550

Figure Lengend Snippet: Strains of H. mediterranei used in reporter gene assays.

Techniques: Mutagenesis

Haloferax mediterranei R4 anaerobic growth curves (circles) and promoter activity (squares) of the four main genes of denitrification ( narGH , nirK , nor, and nosZ ) without the availability of alternative electron acceptors. Promoter activity was measured using the β-galactosidase specific activity. Dotted lines represent the switch from oxic conditions to anoxic. Titles of each graph represent the specific strain used for that study. pVA513 refers to the plasmid used that carries the reporter gene ( bgaH gene). narp (nitrate reductase promoter), nirp (nitrite reductase promoter), norp (nitric oxide reductase promoter), and nosp (nitrous oxide reductase promoter) refer to the promoter cloned in the plasmid. Nov: novobiocin resistance.

Journal: Frontiers in Microbiology

Article Title: Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei

doi: 10.3389/fmicb.2023.1109550

Figure Lengend Snippet: Haloferax mediterranei R4 anaerobic growth curves (circles) and promoter activity (squares) of the four main genes of denitrification ( narGH , nirK , nor, and nosZ ) without the availability of alternative electron acceptors. Promoter activity was measured using the β-galactosidase specific activity. Dotted lines represent the switch from oxic conditions to anoxic. Titles of each graph represent the specific strain used for that study. pVA513 refers to the plasmid used that carries the reporter gene ( bgaH gene). narp (nitrate reductase promoter), nirp (nitrite reductase promoter), norp (nitric oxide reductase promoter), and nosp (nitrous oxide reductase promoter) refer to the promoter cloned in the plasmid. Nov: novobiocin resistance.

Techniques: Activity Assay, Plasmid Preparation, Clone Assay

Journal: Frontiers in Microbiology

Article Title: Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei

doi: 10.3389/fmicb.2023.1109550

Figure Lengend Snippet: Haloferax mediterranei R4 anaerobic growth curves (circles) and promoter activity (squares) of the four main genes of denitrification ( narGH , nirK , nor, and nosZ ) in defined media supplemented with DMSO as an electron acceptor. Promoter activity was measured using the β-galactosidase specific activity. The dotted lines represent the switch from oxic conditions to anoxic. Titles of each graph represent the specific strain used for that study. pVA513 refers to the plasmid used that carries the reporter gene ( bgaH gene). narp (nitrate reductase promoter), nirp (nitrite reductase promoter), norp (nitric oxide reductase promoter), and nosp (nitrous oxide reductase promoter) refer to the promoter cloned in the plasmid. Nov: novobiocin resistance.

Techniques: Activity Assay, Plasmid Preparation, Clone Assay

Journal: Frontiers in Microbiology

Article Title: Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei

doi: 10.3389/fmicb.2023.1109550

Figure Lengend Snippet: Haloferax mediterranei R4 anaerobic growth curves (circles) and promoter activity (squares) of the four main genes of denitrification ( narGH , nirK , nor, and nosZ ) in defined media supplemented with nitrate as an electron acceptor. Promoter activity was measured using the β-galactosidase specific activity. The dotted lines represent the switch from oxic conditions to anoxic. Titles of each graph represent the specific strain used for that study. pVA513 refers to the plasmid used that carries the reporter gene ( bgaH gene). narp (nitrate reductase promoter), nirp (nitrite reductase promoter), norp (nitric oxide reductase promoter), and nosp (nitrous oxide reductase promoter) refer to the promoter cloned in the plasmid. Nov: novobiocin resistance.

Techniques: Activity Assay, Plasmid Preparation, Clone Assay

Journal: Frontiers in Microbiology

Article Title: Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei

doi: 10.3389/fmicb.2023.1109550

Figure Lengend Snippet: Haloferax mediterranei R4 anaerobic growth curves (circles) and promoter activity (squares) of the four main genes of denitrification ( narGH , nirK , nor, and nosZ ) in defined media supplemented with nitrite as an electron acceptor. Promoter activity was measured using the β-galactosidase specific activity. The dotted lines represent the switch from oxic conditions to anoxic. Titles of each graph represent the specific strain used for that study. pVA513 refers to the plasmid used that carries the reporter gene ( bgaH gene). narp (nitrate reductase promoter), nirp (nitrite reductase promoter), norp (nitric oxide reductase promoter), and nosp (nitrous oxide reductase promoter) refer to the promoter cloned in the plasmid. Nov: novobiocin resistance.

Techniques: Activity Assay, Plasmid Preparation, Clone Assay

Haloferax mediterranei R4 aerobic growth curves (circles) and promoter activity (squares) of the four main genes of denitrification ( narGH , nirK , nor, and nosZ ) in defined media supplemented with nitrate as electron acceptor. Promoter activity was measured using the β-galactosidase specific activity. Below each growth graph, it is displayed the measurements of different parameters of the media: oxygen (diamond), nitrate (hexagon), and nitrite (triangles). Titles of each graph represent the specific strain used for that study. pVA513 refers to the plasmid used that carries the reporter gene ( bgaH gene). narp (nitrate reductase promoter), nirp (nitrite reductase promoter), norp (nitric oxide reductase promoter), and nosp (nitrous oxide reductase promoter) refer to the promoter cloned in the plasmid. Nov: novobiocin resistance.

Journal: Frontiers in Microbiology

Article Title: Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei

doi: 10.3389/fmicb.2023.1109550

Figure Lengend Snippet: Haloferax mediterranei R4 aerobic growth curves (circles) and promoter activity (squares) of the four main genes of denitrification ( narGH , nirK , nor, and nosZ ) in defined media supplemented with nitrate as electron acceptor. Promoter activity was measured using the β-galactosidase specific activity. Below each growth graph, it is displayed the measurements of different parameters of the media: oxygen (diamond), nitrate (hexagon), and nitrite (triangles). Titles of each graph represent the specific strain used for that study. pVA513 refers to the plasmid used that carries the reporter gene ( bgaH gene). narp (nitrate reductase promoter), nirp (nitrite reductase promoter), norp (nitric oxide reductase promoter), and nosp (nitrous oxide reductase promoter) refer to the promoter cloned in the plasmid. Nov: novobiocin resistance.

Techniques: Activity Assay, Plasmid Preparation, Clone Assay

A tentative model for denitrification regulatory signals in H. mediterranei R4. In red are the signals that have been assessed in this paper, that complement the first proposed model. The activation of respiratory nitrate reductase transcription by NO has been proposed in this paper (dot line), but it should be analyzed with further studies. Adapted from .

Journal: Frontiers in Microbiology

Article Title: Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei

doi: 10.3389/fmicb.2023.1109550

Figure Lengend Snippet: A tentative model for denitrification regulatory signals in H. mediterranei R4. In red are the signals that have been assessed in this paper, that complement the first proposed model. The activation of respiratory nitrate reductase transcription by NO has been proposed in this paper (dot line), but it should be analyzed with further studies. Adapted from .

Techniques: Activation Assay

β-galactosidase relative specific activity measured in the different H. mediterranei R4 mutants analyzed in site-directed mutagenesis experiments (comparisons against non-mutated control strain). Cultures were grown under anaerobic conditions without an electron acceptor and measurements were taken after 24 h of anoxic growth. (A) nos promoter mutants, (B) nar promoter mutants, (C) nir promoter mutants, and (D) nor promoter mutants. * Conditions with significative differences compared to control (value of p < 0.05).

Journal: Frontiers in Microbiology

Article Title: Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei

doi: 10.3389/fmicb.2023.1109550

Figure Lengend Snippet: β-galactosidase relative specific activity measured in the different H. mediterranei R4 mutants analyzed in site-directed mutagenesis experiments (comparisons against non-mutated control strain). Cultures were grown under anaerobic conditions without an electron acceptor and measurements were taken after 24 h of anoxic growth. (A) nos promoter mutants, (B) nar promoter mutants, (C) nir promoter mutants, and (D) nor promoter mutants. * Conditions with significative differences compared to control (value of p < 0.05).

Techniques: Activity Assay, Mutagenesis, Control

Genes encoding copper membrane transporters found in Haloferax mediterranei.

Journal: Frontiers in Microbiology

Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation

doi: 10.3389/fmicb.2022.895296

Figure Lengend Snippet: Genes encoding copper membrane transporters found in Haloferax mediterranei.

Article Snippet: Regarding the bioinformatic analysis of H. mediterranei genome (strain ATCC 33500), the list of functional genes available in HaloWeb was reviewed.

Techniques: Membrane

Genes coding for cupredoxin-containing proteins found in Haloferax mediterranei genome.

Journal: Frontiers in Microbiology

Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation

doi: 10.3389/fmicb.2022.895296

Figure Lengend Snippet: Genes coding for cupredoxin-containing proteins found in Haloferax mediterranei genome.

Article Snippet: Regarding the bioinformatic analysis of H. mediterranei genome (strain ATCC 33500), the list of functional genes available in HaloWeb was reviewed.

Techniques: Plasmid Preparation

Genes coding for copper metallochaperones in Haloferax mediterranei genome.

Journal: Frontiers in Microbiology

Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation

doi: 10.3389/fmicb.2022.895296

Figure Lengend Snippet: Genes coding for copper metallochaperones in Haloferax mediterranei genome.

Article Snippet: Regarding the bioinformatic analysis of H. mediterranei genome (strain ATCC 33500), the list of functional genes available in HaloWeb was reviewed.

Techniques:

Antioxidant proteins found in H. mediterranei .

Journal: Frontiers in Microbiology

Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation

doi: 10.3389/fmicb.2022.895296

Figure Lengend Snippet: Antioxidant proteins found in H. mediterranei .

Article Snippet: Regarding the bioinformatic analysis of H. mediterranei genome (strain ATCC 33500), the list of functional genes available in HaloWeb was reviewed.

Techniques:

Overview on H. mediterranei copper uptake and efflux transporters that might be involved in copper transport. (IM: Inner membrane). Considering that some genes involved in siderophores synthesis have been identified, potential siderophores are also presented as they contribute to facilitating the uptake of metals. CopG is also represented as the converter of copper (I; black dot) into copper (II; grey dot) and vice versa.

Journal: Frontiers in Microbiology

Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation

doi: 10.3389/fmicb.2022.895296

Figure Lengend Snippet: Overview on H. mediterranei copper uptake and efflux transporters that might be involved in copper transport. (IM: Inner membrane). Considering that some genes involved in siderophores synthesis have been identified, potential siderophores are also presented as they contribute to facilitating the uptake of metals. CopG is also represented as the converter of copper (I; black dot) into copper (II; grey dot) and vice versa.

Article Snippet: Regarding the bioinformatic analysis of H. mediterranei genome (strain ATCC 33500), the list of functional genes available in HaloWeb was reviewed.

Techniques: Membrane

Denitrification pathway described from H. mediterranei . In yellow, copper-containing enzymes NirK and NosZ are represented (NarGH: Nitrate reductase; NirK: Nitrite reductase; qNor: Nitric oxide reductase; NosZ: Nitrous oxide reductase).

Journal: Frontiers in Microbiology

Article Title: Assessment of Haloferax mediterranei Genome in Search of Copper-Molecular Machinery With Potential Applications for Bioremediation

doi: 10.3389/fmicb.2022.895296

Figure Lengend Snippet: Denitrification pathway described from H. mediterranei . In yellow, copper-containing enzymes NirK and NosZ are represented (NarGH: Nitrate reductase; NirK: Nitrite reductase; qNor: Nitric oxide reductase; NosZ: Nitrous oxide reductase).

Article Snippet: Regarding the bioinformatic analysis of H. mediterranei genome (strain ATCC 33500), the list of functional genes available in HaloWeb was reviewed.

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